Genomics Core Director
Phone: (225) 763-2653
Fax: (225) 763-0274
Director: J. Michael Salbaum
Genomics Core Facility
6400 Perkins Road
Baton Rouge, LA 70808
Please send all questions or inquies via email to firstname.lastname@example.org
About the Genomics Core
The Genomics Core Facility seeks to achieve high quality research data production through education of core facility users, optimal use of qPCR and RNA/DNA quality and quantity instrumentation, and quality Sanger sequencing, next-generation sequencing, robotics, and microarray services. Services are provided to internal and external researchers.
The Genomics Core Facility (GCF) provides the following:
- Sanger and next-generation DNA sequencing
- DNA fragment analysis
- qualitative and quantitative analysis of DNA, protein, and RNA samples
- quantitative PCR
- microarray RNA labeling, hybridization, and scanning
- bioinformatics services
Individual and small group training and consultation services are offered for sequence analysis, real-time PCR, next-generation sequencing and microarray analysis.
The GCF laboratory services are available to any researcher. The goal of the GCF is to provide high quality service at low cost to facilitate research. Use of the Genomics Core Facility services are scheduled on a first come, first serve basis. The wait time for instrument use is rarely more than one day and is frequently shorter.
The core laboratory is approximately 900 square feet. Two ancillary laboratories of approximately 168 square feet each house microarray hybridization, RNA and DNA library preparation, and next-generation sequencing. Laboratories are supplied with emergency power with dedicated circuits for instrumentation as well as ultrapure water, gas, air, and vacuum supplies.
The laboratory is equipped with:
- Applied Biosystems 3130XL: Two sixteen capillary genetic analyzers that are used to perform DNA sequencing and fragment analysis. They will accommodate 96 well and 384 well format sample plates.
- Applied Biosystems SOLiD: A next-generation sequencer that sequences DNA or cDNA libraries created for resequencing, methylation studies, chromatin Immunoprecipitation (CHiP) assays, small RNA, 5’ SAGE, and whole transcriptome gene expression analysis.
- Applied Biosystems 7900HT: Four Sequence Detection Systems equipped with 96 well, 384 well, and low density array blocks are available for quantitative PCR.
- Illumina microarray system: This instrument is used to provide microarray service. Commercial arrays in standard microscope slide format can be scanned using a Perkin-Elmer ScanArray 5000. Spotfire software and Bead Studio are used for analysis of microarray data.
- Becton Dickinson Biomek FXand Perkin Elmer MultiProbe II: Two pipetting robots are available for robotic liquid handling. These instruments facilitate high-throughput pipetting of 384 well format plates. They can be programmed for large pipetting projects.
- Qiagen Qiacube: This robot will process up to twelve samples using Qiagen Spin column kits for sample isolation and purification. For large DNA extraction and purification projects, a Qiagen AutoPure robot is used.
- Agilent 2100 Bioanalyzer: Two instruments are used for protein, DNA, and RNA analysis and quantitation.
- NanoDrop Spectrophotometer: This instrument is used for RNA and DNA quantification.
- Qubit Fluorometer: This instrument is used for RNA and DNA quantification.
- Odyssey Infrared Imager: This instrument uses direct infrared fluorescence detection for western blot analysis.
- Seahorse Bioscience XF-24 analyzer: This instrument is capable of measuring the rate of change of analytes (currently dissolved oxygen and pH) in the media surrounding living cells.
- Three computer workstations are available for sequence analysis and alignment, PCR primer design, and RT-PCR data analysis.